CureZone   Log On   Join
 

Re: DMPS chelates both mercury and lead by #20928 ..... Chelation: Andy Cutler Protocol Forum

Date:   2/10/2009 12:54:10 PM ( 12 years ago ago)
Hits:   14,519
URL:   https://www.curezone.org/forums/fm.asp?i=1354055

0 of 0 (0%) readers agree with this message.  Hide votes     What is this?

Yeah, I thought the main purpose when they developed DMSA was to chelate lead?


here's something I should have posted here earlier, but I doubt this is news to Newport. The use of calcium and ascorbic acid along with DMSA.

From Here: http://www.springerlink.com/content/82n737633756070m/



Yingjun Liao1, Jun Zhang2, Yaping Jin3 , Chunwei Lu3, Gexin Li3, Fei Yu4, Xuping Zhi4, Li An4 and Jun Yang4

(1) Department of Physiology, School of Basic Medicine, China Medical University, Shenyang, P.R. China
(2) Department of Public Health Management, School of Professional Technology, Liaoning College of Traditional Chinese Medicine, Shenyang, P.R. China
(3) Department of Environmental and Occupational Health, School of Public Health, China Medical University, No. 92 Beier Road, Heping District, Shenyang, Liaoning, 110001, P.R. China
(4) Department of Nutrition and Food Hygiene, China Medical University, Shenyang, P.R. China
Received: 4 August 2006 Accepted: 18 January 2007 Published online: 8 February 2007

Abstract The aim of this study was to explore the therapeutic efficacies of combined use of meso-2,3-dimercaptosuccinic acid (DMSA) with calcium and ascorbic acid in the treatment of mild to moderately lead-intoxicated mice. Female albino mice were exposed to lead by drinking water contaminated with 0.1% (moderate lead exposure) or 0.05% (mild lead exposure) lead acetate. After the cessation of lead exposure, mice were supplemented by gavage with saline solution, 50 mg/kg body weight (b.w) DMSA, 100 mg/kg b.w DMSA, calcium and ascorbic acid, or 50 mg/kg b.w DMSA and calcium as well as ascorbic acid, respectively. Atomic absorption spectrophotometric method was used to analyze lead levels in blood, bone, liver, kidney and brain. Activities of blood δ-aminolevulinic acid dehydratase (ALAD) were determined by colorimetric method. DMSA supplemented alone could reduce lead levels in both soft tissues and bone and reverse lead-inhibited activities of blood ALAD in mild to moderately lead-intoxicated mice. On the other hand, combined use of DMSA with calcium and ascorbic acid achieved better therapeutic efficacies in mobilizing lead in blood, liver and kidney, and reversing lead-inhibited activities of blood ALAD in moderately lead intoxicated mice than DMSA supplemented alone. Moreover, the better therapeutic efficacies were also found in mildly lead intoxicated mice in mobilizing lead in blood and bone achieved by combined use of DMSA with calcium and ascorbic acid. Combined use of DMSA with calcium and ascorbic acid seems to be the better choice in the treatment of mild to moderate lead-intoxication.
Keywords Lead intoxicated mice - Meso-2,3-dimercaptosuccinic acid (DMSA) - Calcium - Ascorbic acid - Lead body burden - δ-Aminolevulinic acid dehydratase (ALAD)


Yaping Jin
Email: jinyp@mail.cmu.edu.cn

References
American Academy of Pediatrics (1995) Committee on drugs. Treatment guidelines for lead exposure in children. Pediatrics 96:155–60

Berlin A, Schaller KH (1974) European Standardized Method for the determination of aminolevulinic acid dehydratase activity in blood. Zeitsch Klin Chem Klin Biochem 12:389–90


Canfield RL, Henderson CR, Cory-Slechta DA, Cox C, Jusko TA, Lanphear BP (2003) Intellectual impairment in children with blood lead concentrations below 10 μg per deciliter. N Engl J Med 348:1517–26


Chen A, Dietrich KN, Ware JH, Radcliffe J, Rogan WJ (2005) IQ and Blood Lead from 2 to 7 Years of Age: Are the Effects in Older Children the Residual of High Blood Lead Concentrations in 2-Year-Olds? Environ Health Perspect 113:597–601


Cremin JD, Luck ML, Laughlin NK et al (2001). Oral succimer decreases the gastrointestinal absorption of lead in juvenile monkeys. Environ Health Perspect 109:613–9


Dalley JW, Gupta PK, Hung CT (1990) A physiological pharmacokinetic model describing the disposition of lead in the absence and presence of L-ascorbic acid in rats. Toxicol Lett 50:337–48


Dawson EB, Evans DR, Harris WA et al (1999) The effect of ascorbic acid supplement on the blood lead levels of smokers. J Am Coll Nutr 18:166–70


Dietrich KN, Ware JH, Salganik M, Radcliffe J, Rogan WJ, Rhoads GG et al (2004) Effect of chelation therapy on the neuropsychological and behavioral development of lead-exposed children after school entry. Pediatrics 114:19–26


Flora SJS, Pande M, Mehta A (2003) Beneficial effect of combined administration of some naturally occurring antioxidants (vitamins) and thiol chelators in the treatment of chronic lead intoxication. Chem Biol Interact 145:267–80


Fullmer CS (1991) Intestinal calcium and lead absorption: effects of dietary lead and calcium. Environ Res 54:159–69


Gurer H, Ercal N (2000) Can antioxidants be beneficial in the treatment of lead poisoning? Free Radical Biology And Medicine 29:927–945


Han S, Pfizenmaier DH, Garcia E (2000) Effects of lead exposure before pregnancy and dietary calcium during pregnancy on fetal development and lead accumulation. Environ Health Perspect 108:527–31


Houston DK, Johnson MA (2000) Does Vitamin C intake protect against lead toxicity? Nutr Rev 58:73–5


Hsu PC, Guo YL (2002) Antioxidant nutrients and lead toxicity. Toxicology 180:33–44


Jin YP, Kobayashi EK, Nogawa KJ et al. (2000) Determination of Urinary Lead Levels in Normal Population by Graphite-furnace Atomic Absorption Spectrophotometry Using Palladium Chloride as the Matrix Modifier. Analytical Letters 33:1409–24


Kalia K, Flora SJ (2005) Strategies for safe and effective therapeutic measures for chronic arsenic and lead poisoning. J Occup Health 47:1–21


Kalra V, Dua T, Kumar V, Kaul B (2002) Succimer in Symptomatic Lead Poisoning. Indian Pediatrics 39:580–5


Lidsky TI, Schneider JS (2003) Lead neurotoxicity in children: basic mechanisms and clinical correlates. Brain 126:5–19


Liebelt EL, Shannon M, Graef JW (1994) Efficacy of oral meso−2,3-dimercaptosuccinic acid therapy for low level childhood plumbism. J Pediatr 124:313–7


Kleszczewska E (2001) Biological role of reactions of L-ascorbic acid with metals. Postepy Hig Med Dosw 55:81–94


Markowitz ME, Sinnett M, Rosen JF (2004) A randomized trial of calcium supplement for childhood lead poisoning. Pediatrics 113:34–9


Miller AL (1998) Dimercaptosuccinic acid (DMSA), a non-toxic, water-soluble treatment for heavy metal toxicity. Altern Med Rev 3:199–207


Nightangle SL (1991) Succimer (DMSA) approved for severe lead poisoning. JAMA 265:1802


Patra RC, Swarup D, Dwivedi SK (2001) Antioxidant effects of α-tocopherol, ascorbic acid and L-methionine on lead induced oxidative stress to the liver kidney and brain in rats. Toxicology 162:81–8


Peterson KE, Salganik M, Campbell C, Rhoads GG, Rubin J, Berger O et al (2004) Effect of Succimer on Growth of Preschool Children with Moderate Blood Lead Levels. Environmental Health Perspectives 112:233–7


Pounds JG, Long GJ, Rosen JF (1991) Cellular and molecular toxicity of lead in bone. Environ Health Perspect 91:17–32


Shalana MG, Mostafab MS, Hassounab MM, Hassab El-Nabic SE, El-Refaied A (2005) Amelioration of lead toxicity on rat liver with Vitamin C and silymarin supplements. Toxicology 206:1–15


Silbergeld EK, Shwartz J, Mahaffey KR (1988) Lead and osteoporosis: mobilization of lead from bone in postmenopausal women. Environ Res 47:79–94


Simon JA, Hudes ES (1999) Relationship of ascorbic acid to blood lead levels. JAMA 281:2289–93


Smith DR, Calacsan C, Woolard D (2000) Succimer and the urinary excretion of essential elements in a primate model of childhood lead exposure. Toxicol Sci 54:473–80


Stangle DE, Strawderman MS, Smith D, Kuypers M, Strupp BJ (2004) Reductions in blood lead overestimate reductions in brain lead after repeated succimer regimens in a rodent model of childhood lead exposure. Environ Health Perspect 112:302–8


Upasani CD, Khera A, Balaraman R (2001) Effect of lead with Vitamins E, C, or Spirulina on malondialdehyde: conjugated dienes and hydroperoxides in rats. Indian J Exp Biol 39:70–4


Varnai VM, Piasek M, Blanusa M, Juresa D, Saric M, Kostial K (2003) Ascorbic acid supplementation does not improve efficacy of meso-dimercaptosuccinic acid treatment in treatment of lead-exposed suckling rats. Pharmacol Toxicol 93:180–5


Varnai VM, Piasek M, Blanusa M (2004) Succimer treatment and calcium supplementation reduce tissue lead in suckling rats. J Appl Toxicol 24:123–8


Wang S, Zhang J (2006) Blood lead levels in children, China. Environ Res 101:412–8


 

<< Return to the standard message view

fetched in 0.28 sec, referred by www.curezone.org/forums/fmp.asp?i=1354055