IN-VITRO GIARDICIDAL EFFECT OF AQUEOUS AND ALCOHOLIC EXTRACTS OF ASAFOETIDA ON GIARDIA LAMBLIA CYST
REZAIEMANESH M.R.*,SHIRBAZOU SH.
* HEALTH RESEARCH CENTER, BAQHYATALLAH UNIVERSITY OF MEDICAL SCIENSES. TEHRAN, IRAN
Background and Aim: Giardia lamblia, Giardiasis causative protozoa, is one of the most common etiologic agents of diarrhea throughout the world especially in Iran. Asafoetida, an oleo-gum-resin (called Anjodan in Farsi) obtained from an Iranian endemic herb, Ferula Assa-foetida has been used in treating of different diseases, particularly parasitic ones. The aim of this study was in-vitro evaluating of the effect of Asafoetida aqueous and alcoholic extracts on Giardia lamblia cysts.
Materials and Methods: Five hundreds ml of each of each of 1.25, 2.5, 5 and 20 mg/ml concentrations of asafoetida aqueous and ethanol extracts was added to 500 ml of purified Giardia cysts, respectively. The mixtures were kept at 4, 24 and 37oC. The Giardicidal activity of the extracts was measured 1, 2, 3, 4 and 5 hour (s) after exposure through 0.1% eosin dye staining and microscopic enumeration method. The gathered data were analyzed by means of one- way variance, Pearson's correlation coefficient and Independent T-test.
Results: The highest Giardicidal effect of A safoetida ethanol extract was 100% at 37oC, belonging to 20 mg/ml and in the 4th hour after experiment, while the maximum Giardicidal effect of Asafoetida aqueous extract was 57.23% at the same temperature and with the same concentration, in the 5th hour. There was a significant difference between Giardicidal effect of ethanol and water extracts with all concentrations and at different whiles and temperatures (P<0.005). Giardicidal effect of both extracts significantly increased due to rising the co ncentration, time and temperature (P<0.0001).
Conclusion: Ethanol and aqueous extracts of asafetida have in-vitro Giardicidal effect on Giardia lamblia cysts. Ethanol extract has more Giardicidal effect.