I didn't answer your post on the parasite Forum because this computer monitor does not show any details in the brown rectangle. When I first started doing lab work we used the Iron hematotoslyn stain but soon used the easier to do Trichrome stain. The details of Giardia and Dientamoeba trophozoites and cysts would show up clearly with the IH and Trichrome but sometimes with Giardia the inner details of the cysts would not stain with the Trichrome. All the Giardia I found were easily identifiable except for once when there were only cysts present which did not take up the stain. I thought they might be Coccidia but sent them off to an expert and they were unstained Giardia cysts. Dientamoeba takes more attention to inner morphology to ID.