To avoid the unwanted adrenal stimulation or absorbing the caffeine/coffee in the regular intestines which would be similar to when you drink it, I have used just 1 1/2 cups of fairly weak brewed coffee per dose and I laid on my right side (most directions say lay on the left side). That way the coffee solution was guaranteed to stay in the rectum and not get into the sigmoid colon or descending colon. The hepatic-hemorrhoidal vein in the rectum transports the caffeine and other active ingredients to the liver. I used the lightest roast I could get in the organically ground coffee at the health food store. I "brewed" it in a modified old-fashioned "cowboy coffee" as I don't own a coffee-maker. Two to three rounded tablespoons of fine or drip grind coffee (I prefer fine)to 2 qts of water. I bring the water to a boil, remove from heat and put the coffee in the pan and put a lid on. Set aside for a couple of hours and then filter. You can store in glass canning jars in the refrigerator. It keeps up to a week. And yes, even this weak, and made this way (which is supposed to make a weaker brew than boiling), I can hear/feel the gallbladder release the bile. Remember, you hold the first dose for 15 minutes, then expel, then take a second dose and hold for 15min and then expel. The two rounds are considered one enema.