Hi 99158. I do not consider myself a scientist in the professional sense, but I am seriously engaged in the acquisition of knowledge spanning many areas of disease pathology where foreign microbes are concerned.
I have been investing in research equipment, as well as doing controlled experiments for about 4 years now.
I am soon to conduct protein analysis of macromolecules such as antibodies, polypeptides etc. using PAGE and regular electrophoresis techniques.
I cannot do PCR, for lack of primers and polymerase, which I cannot obtain for obvious reasons. I do, however, fully understand the procedure.
I may be able to obtain my own restriction enzymes [endonucleases] from ecoli bacteria--in time.
As for understanding the micro-terrain of the human gut, I am very confident in my ability to spot anything unusual, this, being based on the study of my own "samples" for 4 years under the microscope. I base things on morphology first, but I have a system I use to recognize aggressive forms of bacteria, fungi, anything parasitic, and sometimes viral. The later is based on rather arbitrary methods of observation.
One of my strengths, is understanding immunological events in the body. Observation of fluids, blood, even puss, can provide many insights to the body's reaction to infectious agents. Immunology is one avenue of my "personal" academic pursuits. I am also studying parasitology, and molecular biotechnology.
This forum is a good place to publish personal research, and to learn about trends in peoples chronic health issues. It also provides a wealth of information about the effectiveness of various treatment protocols.
I do research through other forums and blogs etc. as well as personal contacts.
I hope this answers your question, please feel free to contact me any time. Shroom
P.S. As for my own parasitic\fungal problem, it is turning out to be a very convoluted and complex phenomenon, but probably not uncommon.
is more of a nuisance than a pathogen. It causes gastro-intestinal imbalances, but more importantly ties into cell signaling issues resulting in immunotolerances as well as allergies.