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Homemade (With ultrasound) Liposomal-Encapsulated Vit C has 5X oral effectiveness and faster delivery into tissue-bed than Vit C via IV!
  • Homemade (With ultrasound) Liposomal-Encapsulated Vit C has 5X oral effectivenes...   RRR ladylove   6y  45,032  
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    Date: 1/14/2010 12:53:37 PM   ( 6y ago )   Hits:   45033
    Status:       RRR [Message recommended by a moderator!]
    Hello Forumfolk,

    I think this is information is of interest to probably every human being on the planet.

    Good Health to All of Us,

    Ladylove :)

    Make Your Own Liposomal Encapsulated Vitamin C

    From: Brooks Bradley
    Sent: Tuesday, August 11, 2009 12:20 PM
    Subject: CS>Liposomal Encapsulation Technology: Vit “C”

    In our recent researches evaluating this technology and, consequently, in searching for possible “process” improvements/modifications which might facilitate the “lay person” an opportunity for a DIY methodology achievable in a home environment—we did achieve some notable progress.

    First, a brief summary of our exploratory activity. Our literature searches revealed several companies actively exhibiting valid capability in this area (LET).
    Typical, and demonstrably capable, is a company named MICROTEK.
    Helpful information is available here.

    One fact became obvious, early on, to wit: The truly striking feature of LET was a NATURALLY-occurring characteristic…… and not a man-made process, that was driving this encapsulation process. That is, this process is a function of an automatic, “natural tendency” of certain substances (e.g. phospholipids in this case) to form tiny vacoules or
    bubbles—called liposomes—-when in a aqueous solution under certain conditions. ”

    The keystone activity is that these liposomes automatically fill themselves with whatever aqueous solution they were in—-before they were formed. “This type of bubble, called a membrane, forms a protective barrier around virtually every cell in the human body.”

    Livon Labs has perfected a process which employs a high-pressure (1700 p.s.i.) discharge system which directs a liquid stream against a forming plate. The high impact forces the phospholipids (soy lecithin in this case) to form liposomes—-so small they require an electrom microscope for viewing. This technology does not create the LET activity….it just enhances it. In our personal researches we have determined the key to exploiting the LET phenomenon appeared to be Livon’s application of intense force in their mixing methodology.

    Enter the “enlightening” moment. Searching for a method of achieving liposomal encapsulation, it occurred to us to explore ultrasonic stimulation as an option. It worked…maybe not quite as well as Livon’s “high tech” brute force approach…but about 70% as well. Plenty efficient for our purposes.

    Our vitamin “C” liposomal encapsulation protocol is as follows:

    Using a small (2 cup) Ultrasonic cleaner, (Item #03305, obtainable from Harbor Freight @ about $30.00), we performed the following:

    1. Dissolved 3 level tablespoons of soy lecithin in 1 cup of water (preferably distilled). Note: This is key to being successful from the start. Mix the lecithin and distilled water in a seal-able quart jar so you can shake/agitate until the lecithin is completely dissolved. You don’t want any lecithin granules visible. Keep agitating until all granules are dissolved.

    2. Dissolved 1 level tablespoon of ascorbic acid powder (Vit. “C”) in 1/2 cup of water.

    3. Poured both solutions together in the ultrasonic cleaner bowl and turned the unit on. Using a plastic straw (leaving the top of the cleaner opened), gently, slowly, stirred the contents. Note: The cleaner will, automatically, self-stop about every 2 minutes. Just push ON button to continue. Repeat for a total of 3 series (6 minutes). By that time the entire solution should be blended into a cloudy, homogeneous, milk-like mixture. The LET solution is now formed. Tip: Pour the dissolved Vitamin C solution into the seal-able quart jar with the dissolved lecithin and shake briefly prior to pouring into the ultra-sonic cleaner. If you dissolved your lecithin first as directed in the note above you will need to close the lid due to bubbling. Simply open the lid frequently and give the mixture a quick stir before closing the lid again.

    4. This protocol furnishes about 12 grams (12000mg.) of vitamin C product. At 70% encapsulation efficiency, 8400 mg would be of the LET type. This solution will keep, acceptably, at room temperature for 3 to 4 days. Refrigerated, it will keep much longer.
    Note: You can use the verification test described in comment #3 below to test the efficacy of your Liposomal Vitamin C. If you followed the tip about completely dissolving the lecithin granules prior to mixing in the ultrasonic cleaner you should have 75%+ encapsulation.

    We use it so fast around our place…there isn’t enough left to be concerned over storage. The “homogenizing effect” is so powerful that after 3 days at room temperature, no precipitation or solution separation appears evident. This type of sequestered vitamin “C” has demonstrated to be, at least 5 times more effective (per volumetric measure) than any other form of orally-ingested vitamin “c”….that we have tested.

    Additionally, it appears to be even more rapid in tissue-bed availability—-than IV applications. An astounding revelation….to us. We estimate the DIY researcher can produce the active LET portion of this solution for 15 cents per gram….as against about $1.00 per gram from commerci! al sources.

    It is my hope that this, limited, explanation of our activities in this area,
    is of some value to our do-it-yourself health-maintenance researchers. In any event, this protocol has demonstrated to be non-toxic and most helpful to OUR RESEARCHERS.

    Sincerely, Brooks Bradley.

    p.s. A larger, more powerful, ultrasonic cleaner is now available at Harbor Freight. Item number 91593. 2+ liters, for about $60.00. Both units have performed quite well for us. Almost as well as our $500.00 lead zirconate titanate, research grade, unit.

    Hi all…After my first batch of LC sat in the fridge over night it separated…wondering if anyone could explain why or what I might have done wrong? Brooks said it would not separate….hum?Best regards…Sandy
    Dear Sandy,My apologies; I neglected to outline the attendant, probable, variations in the protocol. What I SHOULD have said in my original post is “The visible, obviously homogenized, portion of the solution”, whenever I made the comment about the stability of the completed, resultant, material.I believe you will gain a little better knowledge of the results you achieved, after reading my most recent comment on an inquiry by Sheila.

    Bottom line -your result was perfectly normal. Interestingly, the meniscus may present at the top…or the bottom…..or not at all. Usually if the initial material combination has not run long enough to incorporate a majority of the lecithin (or there is simply too much lecithin for the available ascorbic acid fraction…..the meniscus will form on the top of the sample within a few minutes after stopping the US agitation.

    If your procedure has run acceptably well and-long enough to homogenize well, any meniscus formation will, generally, present on the BOTTOM after overnight storage with or without refrigeration.

    In any event, you are doing fine. If you do not want to consume the isolated lecithin fraction you are observing, just decant the homogenized liposome solution and dispose of the isolated lecithin fraction.

    I hope this information helps your dilemma.

    Sincerely, Brooks Bradley.

    p.s. One just needs to continue to experiment “around-the-edges” of this protocol, in order to achieve optimum results. Do not be reluctant to do such this IS NOT ROCKET SCIENCE …just common sense.

    Dear Sheila,

    Your question has been asked by others….(private inquires addressed directly to me). In the interest of saving me time and energy, I offer the following explanation.

    First, soy lecithin is a slow incorporator, when introduced into aqueous mediums….sometimes. Especially, when there is a high lecithin granule population ratio-relative to the total water volume. The general reaction is that a major percentage of the lecithin blends readily with the the water medium, but there will remain a definitive lecithin component which floats on the surface and exhibits a somewhat “gelatinous” appearance (this is quite natural, based upon the native characteristics of the substances involved). Do not fret over encountering such circumstances……they will not compromise the basic effectiveness of your protocol.

    However, it is of some import to understand that the speed, and completeness, of the incorporation of the granular lecithin—into the aqueous medium, is affected by a number of conditions such as the total amount of lecithin versus the total volume of water; the temperature of the water-based solution and the strength of any other substance being incorporated into the parent solution-from very weak, to saturated (none of which are seriously compromising).

    Under the best of conditions, even after ultrasonic mixing for 8 to 9 minutes….there is, often, a thin meniscus (a distinct separation between two or more liquids in the same container). [Example: a thin layer of oil lying on top of water.] In the liposome generation methodology we are discussing, the visible, gelatinous, portion of the meniscus is principally made up of unincorporated lecithin. Is IS NOT a problem, in fact the lecithin component has useful, cardiovascular, health-support effects-beyond those being discussed here.

    Either (or both) of two measures may be executed to reduce the volume of unincorporated lecithin you may be encountering. First, increasing the volume of the total water fraction, or secondly, raising the temperature of the total parent solution and extending the time of US reaction exposure.

    One reason for the condition you are encountering is that the closer one gets to achieving a saturated solution of lecithin….the more resistant the process becomes to accepting more granular lecithin into that solution until the point is reached where no further material will incorporate hence, THE SATURATION POINT IS EXPERIENCED.

    In my brief, original post, I did not discuss the nuances of speed, degree or completeness of dissolution of the lecithin—-or for that matter— the ascorbic acid fraction. Neither did I outline a number of other considerations; such as the effects of varying the volume of water versus the ratios of the solution components ..or the total water volume versus the protocol components primarily, because such elaborations would not serve usefulness/effectivity for the nontechnical.

    DIY person. I simply outlined a SAFE, mid-spectrum, protocol allowing the average lay-person to achieve a measure of acceptable results for home experimental research.

    My personal bias is that it is better to have a small, uncombined, lecithin fraction presenting as a meniscus…..than to strive toward what I perceive to be a cosmetic achievement-of small means of diluting the total solution. In any event the excess lecithin is a positive is just not active in the liposome process until some parameter changes that avails it the opportunity participate in the encapsulation process.

    My final comment on this subject: If it is of paramount importance to one,regardless of reason. by just increasing the water volume and reactivating the US Cleaner for several minutes….the remaining lecithin will (in almost all cases) go into the emulsified solution. However, bear in mind, you have diluted the entire solution by an equivalent strength with NO increase in total vitamin C component.

    Please understand, these comments are not meant to browbeat “anyone”….in any way….but, rather, to aid the less technically-informed on the list.

    Sincerely, Brooks Bradley.

    This entry was posted on 11. August 2009 at 15:26 and is filed under Supplements. You can follow any responses to this entry through the RSS 2.0 feed. You can leave a response or trackback from your own site.

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    13 Responses to “Make Your Own Liposomal Encapsulated Vitamin C”
    admin says:
    14. August 2009 at 21:43
    Hello Everyone…

    Today I bought everything I needed to make my Liposomal Vit-C. My ultrasonic cleaner was on sale so that was a plus…$24.95. I put everything together just like Brooks said and it all turned out the way he said would…so I took my first dose…the taste was not bad at all. I also bought some d-ribose powder and plan to make that into a liposomal concoction. I cannot wait to feel the results.

    Best regards…


    admin says:
    16. August 2009 at 16:03
    From the Silver List.

    Although not scientifically rigorous, I offer a simple test which will yield the DIY researcher some element of confidence that they do, in fact, have a useful measure of liposomal encapsulate.

    First, pour about 4 ounces of your finished Vitamin C encapsulate into a cylindrical, 12 ounce water glass. Next, place 1/4 teaspoon of sodium bicarbonate into about 1 ounce of distilled water and stir for 3 to 5 seconds. Next, pour the sodium bicarbonate solution into the Vitamin C mixture and stir gently for several seconds.

    Note: If the foam/bubble line which forms on top is 1/2 inch or less—in height—you have about a 50% encapsulation efficiency. If the foam/bubble line is 3/8 of one inch…or less, you have about a 60% efficiency.

    If the foam/bubble line is 1/8 inch or less, you have about 75% efficiency. If the foam/bubble line is just a trace… should major in chemistry.

    The percentages given above, represent the amount of the total Vitamin C component incorporated during the encapsulation process…..that was actually encapsulated. The less encapsulation….the greater the foaming.

    What is, actually, occurring in this test is that the ascorbic acid fraction is being transformed into the sodium ascorbate form of vitamin C. This test does not negatively affect the usefulness of the solution you have tested… the isolated Vitamin C component is not adversely affecting the encapsulate (which is being protected by the lecithin bubble-covering.) Actually, the sodium ascorbate form of vitamin C is greater than an order-of-magnitude more soluble for tissue incorporation……than is the ascorbic acid form.

    In any event this simple test should serve to raise the level of confidence in the DIY researcher…. that they do—in fact—have a useful measure of encapsulated vitamin C.
    Sincerely, Brooks Bradley.

    p.s. I had, a few moments ago, just finished a much more extensive posting…..but some form of invasive advertising spam flashed across the top of my mail system and in attempting to circumvent/nullify the invader

    I lost my entire post. The actual post your are receiving is the product of my existing dismay. — The Silver List is a moderated forum for discussing Colloidal Silver.

    admin says:
    19. August 2009 at 16:18
    Just to recapitulate (please correct me if I ‘ve got anything wrong):

    1) + 2) The frequency and power of the ultrasonic cleaner (UC) seems to make not so much of a difference, and can be adjusted by increasing the duration of the dissolving process in the cleaner. Either a 50 watts, 42,000 cycles, $29.00, or a 160 watts, five preset cycles, $69.99 would work almost as well as a $500.00 lead zirconate titanate, research grade, unit.

    3) As a source of phospholipids the dried granular form seems to give better results than liquid soya lecithin. About 3 level tablespoons of lecithin granules should be dissolved into 1 cup of distilled water.

    4) The ratio of pure ascorbic acid powder to distilled water should be 1 level tablespoon (12 grams) in 1/2 cup of distilled water dissolved separately.

    5) Both solutions are next poured in the UC - better in a separate glass due to concerns of corrosion of the stainless steel container - and slowly stirred while the cleaner is turned on. After about 6 minutes of ultrasonic dissolving the entire solution should be blended into a cloudy, homogeneous, milk-like mixture. Ready for oral use.

    6) “The visible, obviously homogenized, portion of the solution” will keep acceptably at room temperature for 3 to 4 days. Refrigerated, it will keep much longer.

    natvlegl says:
    26. August 2009 at 17:23
    Am I understanding correctly that encapsulating Vit C liposomatically will multiply the amount of C exponentially … like, grow more?? That is my understanding from reading it. Or does it just make the encapsulated C more bioavailable? Thanks for clarifying. ALSO … IF it does somehow multiply the amount of C by the process, would this also work with other water-soluble supplements?

    Instead of soy lecithin, would polysaccharides of various kinds work to liposomatically encapsulate them also?

    Thanks for your answer.

    drhiii says:
    26. August 2009 at 18:48
    I like this technique a great deal. 7 years ago met Dr. Chen of Sunrider and he discussed his use of infusion of vitamins into food sources, making them bio available to the body. It was a very cool discussion, and also one that I did not know how to replicate.

    This appears to be on the same plane. I’ve hoped to see this for a long, long time. So, kudos, and I will be participating in this going forward.

    Oh, means I went out and acquired an Ultrasonic machines, some non GMO lecithin, ascorbic acid, and created my first brew. It worked. I sensed it worked very well. Ingestion of results, and one could sense this was a very cool protocol.

    Loaded and mebbe premature question… what else does the author feel can be LET’d? Would lecithin remain the carrier or transport layer in this? And could say, minerals or similar things be infused?

    This is great science, or not science as the OP said, but it makes sense and my sense of the first results are very, very interesting. Me like…

    mikeleekc says:
    2. September 2009 at 22:16
    I noticed several websites offering many types liposomal supplements apart from Vit C.
    This got me thinking whether I can use the same method outlined here to encapsulate other supplements like curcurmin/tumeric powder which has a very low bioavailability?

    Best Regards,

    leone says:
    13. September 2009 at 16:06
    I have made several preps of lipo C ( using USC 2.5 liter model 95563 Chicago Electric Power Tools from Harbor Freight) as described by Brooks and all have worked well. I have tried several modifications:
    1. increased the lecithin ( minimum 95% phosphatides) from 3 to as high as 6 tablespoons( 42.0 grams)
    2. have used a power hand mixer with “wire beater” to mix lecithin…appears to “soluibilize” the lecithin much faster than the shaking time in a bottle
    3. have used sodium ascorbate,calcium ascorbate and ascorbic acid…sometimes as a combo…amounts 16.6 to 89.0 grams
    4.with high amounts of C I have used 4-5 cycles of 180 sec each( started with 90 sec but increased the last few)… have measured surface temp with digital laser thermometer…84-88 degrees F at the end of 5th cycle ( have not used heater)
    5. have kept USC cover on last number of preps with 1 or 2 stirrings
    Purchased a commercial liposome prep…8 oz bottle with 10 gm C/ounce…very syrupy
    I have assumed that the commercial prep is getting a greater conc of lecithin to get
    that high of a concentration of C
    6. stored all preps in the refrigerator

    admin says:
    4. October 2009 at 11:21
    Those reading this subject will further benefit from the discussion on Liposomal Vitamin C Absorbate accessed from the links on the left.


    girma1 says:
    20. October 2009 at 00:36
    Mike asked about possibly using the liposomal method above for encapsulating curcumin. I wondered too because of all the research and extremely positive outcomes for curcumin use. The problem is its very low bioavailability and insolubility in water.

    So I’ve tried using the same lecithin and water mixture as with the liposomal vitamin C, then adding a tablespoon of curcumin powder to a warmed 1/2 cup of olive oil and heating further until the mixture suddenly goes clear (prior to boiling). Then I follow the same protocol as with the liposomal vitamin C: mix and blend the lecithin mixture and the curcumin-olive oil mixture together, and put the mixture through the three rounds of ultra-sound. The result is what appears to be a granule free liquid that can be poured onto or into almost any food you might choose. It keeps well in the fridge and hasn’t given me any trouble at all. The only difficulty of course is managing to keep free of the strong stain if you splash or spill any of the mixture.

    I have no idea how to test the method for effective encapsulation. If anyone has a suggestion, please post.

    cherye7 says:
    21. October 2009 at 19:08
    Hi. I’m sensitive to all forms of Vit. C except ascorbyl palmitate. If ascorbic acid bothers my stomach, will it still bother me after I put it in the UC? Will ascorbyl palmitate be OK to use instead? Thanks!

    admin says:
    6. November 2009 at 15:55
    Several others that had stomach problems with ascorbic acid found they had no problems taking their homemade liposomal Vitamin C. Best advice I can give is to try it. If you don’t want to buy the ultra-sonic unit without knowing try one of the commercial brands first.
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